UCO powers carrier from Singapore to South Africa
This helped reduce the vessel's carbon dioxide emissions by 5%.
The Frontier Jacaranda made the journey with a mix of 7 per cent biofuel and 93% regular fuel.
Alpha Biofuels blended the biofuel at their plant in Tuas, converting it from UCO collected from food manufacturers, food and beverage businesses and households around the island.
A company statement said: "The operation was instrumental in verifying the stability of the biofuel in storage and its performance as a fuel.
"(It is important) that the vegetable oils Singapore cooks with at its Unesco World Heritage-listed hawker centre stalls, along with its highly regarded restaurants, doesn't go down the drain into grease traps. It is in demand locally and globally as a clean transport fuel."
Frontier Jacaranda is owned by Japanese shipping company NYK Line. Alpha Biofuels supplied the fuel to Toyota Tsusho Petroleum, which used the Singapore-registered bunkering ship Marlin Tiga to feed the bigger carrier with the biofuel on June 11.
Alpha Biofuels chief executive and founder Allan Lim said the company should not have a problem sourcing UCO and that it would consider looking to Malaysia and Indonesia if demand grew.
"We do need to make sure the used cooking oil is verified as waste. It must not be contaminated in any way or adulterated with fresh cooking oil. Quality control is important in the collection, processing and supply chain," he said.